List of top Life Science Questions on Techniques

Select the correct statements for D gel electrophoresis:
A. Small D fragments remain near to the well
B. Longer D fragments move towards the negative end of the gel
C. Longer D fragments remain near to the well
D. Smaller D fragments move towards the positive end of the gel
For identification of desired D by Southern blotting, select the correct order
A. Blotting
B. Probe binding
C. Autoradiogram
D. Electrophoresis
E. Gel treatment
Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: 12-20% acrylamide in PAGE restricts the migration of large molecules.
Reason R: Pore size of the gel affects the separation of molecules.
Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: Spectroscopy technique is used for the study of interaction of electromagnetic radiation with matter.
Reason R: It is used for the identification of substances through the spectrum emitted or absorbed by them.
In animal cell culture, sodium bicarbote is added in culture media to:
To detect specific proteins in a sample we use:
The most commonly used stain for visualising/observing the D fragments is:
R$_f$ value depends on:
Which of the following is not true about gel electrophoresis?
The portion of transmembrane proteins that cross the lipid bilayer are usually:
Which of the following is not a fluorescence dye?
The technique used to identify the specific DNA in bacterial colonies is:
Which metal is used for electron beam generation in scanning electron microscopy?
What is the role of sodium dodecyl sulfate (SDS) in protein electrophoresis?
(A) Add net negative charge to the protein
(B) Separate lipids on the basis of their molecular weight
(C) Modify native conformation of a protein
(D) Allows direct destaining of the proteins
Choose the correct answer from the options given below:
Isoelectric focusing allows separation of the proteins according to their:
Match List I with List II:
List IList II
(A) Western Blotting(II) Protein identification
(B) Southern Blotting(I) DNA identification
(C) Northern Blotting(IV) RNA identification
(D) Yeast two-hybrid system(III) Protein-protein interaction
Choose the correct answer from the options given below:
Arrange the given steps involved in polymerase chain reaction (PCR):
(A) Denaturation
(B) Annealing
(C) Extension
(D) Cycling
Choose the correct answer from the options given below:
Vertical gel electrophoresis is routinely used for the separation of:
Which is not a unit of radioactivity?
In the technique called centrifugation, the centrifugal field (G) is calculated as: