Question

Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R
Assertion A: 12-20% acrylamide in PAGE restricts the migration of large molecules.
Reason R: Pore size of the gel affects the separation of molecules.

• A. Both A and R are correct and R is the correct explation of A
• B. Both A and R are correct but R is NOT the correct explation of A
• C. A is correct but R is not correct
• D. A is not correct but R is correct

Hint:

High % Acrylamide (12–20%) = Small pore size $\to$ Best for separating SMALL molecules (restricts large ones).
Low % Acrylamide (4–7.5%) = Large pore size $\to$ Best for separating LARGE molecules.

Answer 1

The Correct Option is A