Question

The technique used to identify the specific DNA in bacterial colonies is:

• A. In-situ hybridization
• B. Dot blot
• C. Colony hybridization
• D. Western hybridization

Hint:

Review the techniques used for identifying specific DNA sequences in bacterial and other microbial colonies.

Answer 1

The Correct Option is C

Colony hybridization is a molecular biology technique used to identify specific DNA sequences within bacterial colonies. The method involves transferring colonies to a membrane, lysing them to free DNA, and then hybridizing with a labeled DNA or RNA probe designed to bind the target sequence. This enables the detection of colonies possessing a gene or sequence of interest.