Question

For identification of desired D by Southern blotting, select the correct order
A. Blotting
B. Probe binding
C. Autoradiogram
D. Electrophoresis
E. Gel treatment

• A. A, E, D, B, C
• B. D, E, A, B, C
• C. E, D, A, B, C
• D. B, A, E, D, C

Hint:

Southern Blotting Protocol Steps:
1. Electrophoresis (D) $\to$ 2. Gel Treatment/Deturation (E) $\to$ 3. Membrane Blotting (A) $\to$ 4. Probe Hybridization (B) $\to$ 5. Autoradiography Detection (C).

Answer 1

The Correct Option is B