List of top Life Science Questions on Biotechnology

Match the LIST-I with LIST-II

Most commonly used technique(s) for development of transgenic animals are:
A. Microprojectile
B. Retrovirus- mediated gene transfer
C. D- pronuclear microinjection
D. Electroporation

Which of the following are True for modifying enzymes used in rD technology?
A. Alkaline phosphatase is used to attach a phosphate group
B. Termil deoxynucleotidyl transferase can remove termil phosphate group
C. Reverse transcriptase is used to generate cD
D. Polynucleotide kise is used to attach a phosphate group

Select the correct order of steps of plant tissue culture:
A. Explant sterilization
B. Selection of media
C. Excision of regenerated shoots
D. Inoculation of explants
E. Transfer of plantlets to sterilised soil

Select the correct order of steps involved in Cloning:
A. Selection
B. Restriction digestion
C. Ligation
D. Transformation
E. Gene of Interest (Insert) and vector

Restriction enzymes commonly used in Recombint D technology are:

cry genes for Bt cotton are obtained from:

Retroviruses are enveloped viruses that can be engineered to deliver how many bases of single-stranded RNA?

Adenoviruses are frequently used in gene delivery experiments. Their average genome size is 36,000 base pairs. Of their total DNA content, up to how much of the adenovirus genome can be replaced by foreign DNA?

Gene gun is one of the popular physical delivery methods. Microparticles that are loaded to deliver DNA are made up of which metal?

Which one of the recombinant DNA techniques is almost obsolete?