Question:medium

Which of the following are True for modifying enzymes used in rD technology?
A. Alkaline phosphatase is used to attach a phosphate group
B. Termil deoxynucleotidyl transferase can remove termil phosphate group
C. Reverse transcriptase is used to generate cD
D. Polynucleotide kise is used to attach a phosphate group

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D Modifying Enzymes Summary:
Alkaline Phosphatase = REMOVES $5'$ phosphate.
Polynucleotide Kise = ADDS $5'$ phosphate.
Termil Transferase (TdT) = ADDS $3'$ homopolymer tails.
Reverse Transcriptase = R $\to$ cD.
Updated On: Jul 28, 2026
  • B and C Only
  • A and C Only
  • C and D Only
  • B and D Only
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The Correct Option is C

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