Question

Select the correct order of steps involved in Cloning:
A. Selection
B. Restriction digestion
C. Ligation
D. Transformation
E. Gene of Interest (Insert) and vector

• A. E, B, D, A, C
• B. E, D, B, C, A
• C. E, B, C, D, A
• D. E, C, D, A, B

Hint:

Cloning Core Steps:
1. D/Vector (E) $\to$ 2. Cut/Digest (B) $\to$ 3. Paste/Ligate (C) $\to$ 4. Transform (D) $\to$ 5. Select (A).

Answer 1

The Correct Option is C