Step 1: Polymerase Chain Reaction (PCR) is an exponential amplification process. Each cycle doubles the quantity of DNA copies.
Step 2: The total number of DNA copies after \( n \) cycles is determined by the formula: \[ \text{Number of copies} = 2^n \]
Calculation: The result of applying the formula for 5 cycles is: \[ 2^5 = 32 \]
Conclusion: Following five PCR cycles, 32 DNA copies are generated, calculated as \( 2^5 = 32 \).
The following diagram shown restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’gens :