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How many DNA copies are produced after five cycles of PCR (Polymerase Chain Reaction)?

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Each PCR cycle exponentially increases the number of DNA copies, making PCR a powerful technique for amplifying specific DNA sequences from small initial quantities.
Updated On: Nov 26, 2025
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Solution and Explanation

Step 1: Polymerase Chain Reaction (PCR) is an exponential amplification process. Each cycle doubles the quantity of DNA copies.
Step 2: The total number of DNA copies after \( n \) cycles is determined by the formula: \[ \text{Number of copies} = 2^n \]

Calculation: The result of applying the formula for 5 cycles is: \[ 2^5 = 32 \]

Conclusion: Following five PCR cycles, 32 DNA copies are generated, calculated as \( 2^5 = 32 \).

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