The correct option is 3: EcoRI
EcoRI, a restriction endonuclease, specifically recognizes and cleaves DNA at or near defined sequences. In contrast, DNA polymerase synthesizes DNA, RNA polymerase synthesizes RNA, and ligase joins DNA fragments.
Answer the following questions:
(a) [(i)] Explain how some strains of Bacillus thuringiensis produce proteins that kill certain insects such as lepidopterans but do not kill the Bacillus.
[(ii)] How is the above mechanism exploited for the production of Bt cotton plant by biotechnologists?
(b) [(i)] Explain how the amplification of gene of interest is done using PCR.
[(ii)] State two applications of the desired amplified fragment of DNA.