Agrobacterium tumefaciens harbors the tumor-inducing (Ti) plasmid, a large extrachromosomal element. This plasmid facilitates the transfer of a DNA fragment into plant cells, inducing crown gall tumor formation. The Ti plasmid serves as a crucial tool in plant genetic engineering for introducing novel genes into plants.
Scientists leverage the Ti plasmid's inherent DNA transfer mechanism to modify plant genomes. By substituting the genes responsible for tumor induction with target genes, researchers can develop genetically engineered plants possessing advantageous characteristics like enhanced pest resistance or improved crop yield.
The following diagram shown restriction sites in E. coli cloning vector pBR322. Find the role of ‘X’ and ‘Y’gens :