List of top Microbiology Questions on Biotechnology

Which of the following is correct about resolving power of a Microscope ? A. Limit of Resolution is directly proportional to the numerical aperture.
B. Resolution power of a microscope increases with magnification.
C. The greatest resolution in light microscopy is obtained with the shortest wavelength of light.
D. Limit of resolution is the smallest distance by which two objects can be separated and still be distinguishable.
E. The maximum resolution of a light microscope is \(0.02\, \mu m\).
Choose the correct answer from the options given below :

Which of the following statement about Fungi are correct ? A. Dimorphic fungi can exist as unicellular yeast like as well as filamentous mold form.
B. Most fungi prefer an alkaline pH for growth.
C. Fungi are eukaryotic, spore bearing, photosynthetic organisms.
D. Conidiospores are sexual spores of fungi.
E. Some fungi can capture nematodes and kill them.
Choose the correct answer from the options given below :

Which of the following statements is correct ? A. Intercalating agents distort DNA to induce single nucleotide pair insertions and deletions.
B. T and G typically exist in the amino form and A and C in the keto form.
C. Base analogues are structurally similar to normal nitrogenous bases.
D. Point mutations are mutations that occur at specific positions.
E. Nonsense mutations convert a sense codon to a stop codon.
Choose the correct answer from the options given below :

Arrange the following steps of root nodule formation in correct order :
A. Formation of bacteroids and differentiation into nitrogen fixing symbiosomes
B. Attachment of bacterial cells to root hairs
C. Plant root hair with attached Rhizobium undergoes curling
D. Bacterial penetration into root hair cell and cell to cell spread of rhizobia
E. Plant root releases flavonoids
Choose the correct answer from the options given below :

Arrange the steps in cloning and expressing a gene in correct order :
A. Introduction of recombinant molecule into new host that expresses the gene
B. Use of restriction enzymes or PCR to generate fragments of DNA
C. Protein purification
D. Isolation of DNA to be cloned
E. Generation of a recombinant molecule by inserting DNA fragments into vectors
Choose the correct answer from the options given below :

Which of the following is not a feature of B-DNA ? A. Right handed double helix
B. 12 base pairs per turn of helix
C. Wide major grooves and narrow minor grooves
D. Anti glycosyl bond conformation at C and syn at G
E. Pitch per turn of helix \(33.2 \AA\)
Choose the correct answer from the options given below :

Arrange the following steps of site-directed mutagenesis in correct order :
A. Transformation of ds DNA into cells
B. Denaturation and annealing of oligonucleotide primers with mutation
C. Repair of nicks in mutated plasmid inside cells
D. Extension and incorporation of primers by using DNA polymerase
E. Digestion of unmutated parental DNA template with methylation specific nuclease and annealing of newly synthesized strands
Choose the correct answer from the options given below :

Arrange the following steps of crossing over in correct order :
A. Pachytene stage
B. Synapsis of homologous chromosomes
C. Separation of homologous chromosomes
D. Breakage and reunion of chromatids
E. Formation of chiasmata
Choose the correct answer from the options given below :

Given below are two statements :
one is labelled as
Assertion (A) and the other is labelled as
Reason (R).
Assertion (A) :
E.coli is widely used as a host in RDT.
Reason (R) :
E.coli grows rapidly and its genetics are well understoo
D.
In the light of the above statements, choose the most appropriate answer from the options given below :

Given below are two statements :
one is labelled as
Assertion (A) and the other is labelled as
Reason (R).
Assertion (A) :
The closer two loci reside along the axis of the chromosome, the more likely they are to be inherited together.
Reason (R) :
The farther apart two linked loci, the more likely a random crossover event will occur in between them.
In the light of the above statements, choose the most appropriate answer from the options given below :

Given below are two statements :
one is labelled as
Assertion (A) and the other is labelled as
Reason (R).
Assertion (A) :
UV spectrophotometry is commonly used to estimate nucleic acids.
Reason (R) :
Nucleic acids absorb UV light maximally at 280 nm.
In the light of the above statements, choose the most appropriate answer from the options given below :

RNAi mediated RNA interference mediates which of the following?

Given below are two statements :
one is labelled as
Assertion (A) and the other is labelled as
Reason (R).
Assertion (A) :
A cDNA library contains only expressed genes.
Reason (R) :
A cDNA library is made from the mRNA molecules of a cell population.
In the light of the above statements, choose the most appropriate answer from the options given below :

Denaturation of proteins involves:

Which of the following restriction endonucleases generates \(3'\) overhangs?

Mendel crossed peas having round green seeds with peas having wrinkled yellow seeds. All \(F_1\) plants had seeds that were round and yellow. What will be genotypic ratio of testcrossing these \(F_1\) plants?

At which side of the transcription unit, most of the RNA polymerase II promoters are located?

Which of the following is not a feature of RNA?

Blue-white screening is based on the activity of: