Question

Arrange the steps in cloning and expressing a gene in correct order :
A. Introduction of recombinant molecule into new host that expresses the gene
B. Use of restriction enzymes or PCR to generate fragments of DNA
C. Protein purification
D. Isolation of DNA to be cloned
E. Generation of a recombinant molecule by inserting DNA fragments into vectors
Choose the correct answer from the options given below :

• A. \(D, B, E, A, C\)
• B. \(A, C, E, B, D\)
• C. \(B, C, A, D, E\)
• D. \(C, E, A, B, D\)

Hint:

Remember the cloning workflow: \[ \text{Isolate DNA} \rightarrow \text{Cut/Amplify} \rightarrow \text{Ligate into Vector} \rightarrow \text{Transform Host} \rightarrow \text{Purify Protein} \]
• Restriction enzymes create compatible DNA ends.
• Vectors commonly used include plasmids and bacteriophages.

Answer 1

The Correct Option is A