Question:medium

Which of the following restriction endonucleases generates \(3'\) overhangs?

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Remember common restriction enzymes:
• EcoRI \(\rightarrow 5'\) overhang
• HindIII \(\rightarrow 5'\) overhang
• PstI \(\rightarrow 3'\) overhang
• SmaI \(\rightarrow\) Blunt ends \[ \text{Sticky ends improve efficiency of DNA ligation} \]

Updated On: Jun 6, 2026

EcoRI
Hind III
Pst I
Sau 3AI

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The Correct Option is C

Solution and Explanation

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List-I	List-II
A. Abscisic acid	I. Promotes female flowers in cucumber
B. Ethylene	II. Helps seeds to withstand desiccation
C. Gibberellin	III. Helps in nutrient mobilisation
D. Cytokinin	IV. Promotes bolting in beet, cabbage etc

List-I	List-II
A. ETS Complex I	I. NADH Dehydrogenase
B. ETS Complex II	IV. Succinate Dehydrogenase
C. ETS Complex III	II. Cytochrome b_c1
D. ETS Complex IV	III. Cytochrome C oxidase

Which of the following restriction endonucleases generates \(3'\) overhangs?

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The Correct Option is C

Solution and Explanation

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