Question

Given below are two statements :
one is labelled as
Assertion (A) and the other is labelled as
Reason (R).
Assertion (A) :
UV spectrophotometry is commonly used to estimate nucleic acids.
Reason (R) :
Nucleic acids absorb UV light maximally at 280 nm.
In the light of the above statements, choose the most appropriate answer from the options given below :

• A. Both (A) and (R) are correct and (R) is the correct explanation of (A)
• B. Both (A) and (R) are correct but (R) is not the correct explanation of (A)
• C. (A) is correct but (R) is not correct
• D. (A) is not correct but (R) is correct

Hint:

Remember: \[ 260 \text{ nm} \rightarrow \text{Nucleic acids} \] \[ 280 \text{ nm} \rightarrow \text{Proteins} \] Purity of DNA is commonly checked using: \[ \frac{A_{260}}{A_{280}} \] A ratio of about \(1.8\) indicates relatively pure DNA.

Answer 1

The Correct Option is C