List of top Life Science Questions on Molecular Genetics

Choose the correct order of steps involved in nucleotide excision repair of damaged bases as their occur.
A. A fresh burst of DNA synthesis
B. Cuts are made on both the 3' side and the 5' side of the damaged area
C. The DNA is unwound producing a "bubble".
D. A DNA ligase covalent binds the fresh piece into the backbone.
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The DNA of the bacterial cell is protected from the cell's own restriction enzymes by the addition of methyl group (-CH3) to ___________ of cytosine.
A. 4th Carbon
B. 7th Carbon
C. 6th Carbon
D. 5th Carbon
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Which one of the following statement is incorrect regarding the work of Erwin Chargaff?

Choose the correct sequence of different regions on a typical eukaryotic DNA segment when we go from the upstream to the downstream of a transcription start point.
A. Initiation site
B. CAAT box
C. Enhancer
D. TATA box
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Match LIST-I with LIST-II

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The left-handed helix, Z-DNA is 18 \AA \ (1.8 nm) in diameter which contains _____________________.

Which of the following statements are correct regarding prokaryotic genomic organization?
A. Most prokaryotes contain a single large circle of double stranded DNA as its genome.
B. The nucleoid is an irregularly shaped region that contains the cell's chromosome and numerous proteins.
C. Nucleoid associated proteins (NAPs) are not particularly important during cell division for the compaction of chromosome.
D. Prokaryotic genomes are much smaller as compared to eukaryotes.
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Choose the correct sequence of events as they occur during a bacterial transcription
A. RNA polymerase sigma (\(\sigma\)) subunits recognizes promoters
B. DNA double helix unwind
C. Sigma (\(\sigma\)) subunit dissociates from the holoenzyme
D. Formation of hairpin secondary structure
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Which of the following are autosomal recessive genetic disorder?
A. Huntington disease
B. Sickle cell anemia
C. Lesch-Nyhan syndrome
D. Tay-Sachs disease
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Match LIST-I with LIST-II

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Which of the following statement is true for Barr body?

Which of the following statement is true for X-linked recessive inheritance?

In the human karyotype, the X chromosome belongs to which group?

While delivering genes by chemical methods, one popular conjecture about what happens to poly(ethylenimine)/DNA complexes following endocytosis is known as the proton sponge hypothesis, which entails the following steps:
(A) PEI/DNA complexes are endocytosed.
(B) V-ATPases will pump protons into the vesicular interior.
(C) Water will enter the vesicle to balance the osmotic gradient.,
(D) The vesicle will swell due to the entry of ions and water, leading to rupture.
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On the basis of type of body cavity, platyhelminthes can be categorized as

Match List I with List II:

LIST I (Condition)	LIST II (Characteristics)
A. Color blindness	I. Insensitivity to green and red light
B. Hemophilia B	II. Deficiency of clotting factor IX
C. Hemophilia A	III. Deficiency of clotting factor VIII
D. G-6-PD deficiency	IV. Severe anemic condition

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Match List I with List II

List I	List II
(A) Radiation hybrid mapping	(I) DNA can be cut into large fragments and circularized for use in chromosome walking
(B) Sequence tagged site (STS) mapping	(II) Useful for cloning of overlapping DNA fragments (restricted to about 200 kb)
(C) Chromosome jumping	(III) Fragment genome into large pieces and locate markers and genes
(D) Chromosome walking	(IV) Applicable to any part of DNA sequence if some sequence information is available

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What percentage of genes are found in the human genome?

Arrange the steps in the correct order as they are involved in RNA interference (RNAi):
(A) Formation of small interfering RNAs (siRNAs)
(B) Activation of RNase III-like enzyme Dicer
(C) Inactivation of target RNA
(D) Formation of RISC complex
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A clotting disorder which is a sex-linked disease:

Match List I with List II

LIST I (Transposons)	LIST II (Organism)
A. Pelement	I. Drosophila
B. LINE and SINE	II. Human
C. Ty element	III. Yeast
D. Tn element	IV. Bacteria

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Arrange the different steps of transcription in the correct order:
(A) Transcription bubble formation
(B) Phosphorylation of CTD tail
(C) Recruitment of elongation factors
(D) Dephosphorylation of CTD tail
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Transcription factors that mediate the binding of RNA polymerase to DNA template are
A. TFIIA
B. TATA box
C. TFIIB
D. Small nuclear ribonucleoproteins
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tRNA is synthesized by:

Which of the following enzyme is defective in galactosemia—a fatal genetic disorder in infants?