Question

Match List I with List II

List I	List II
(A) Radiation hybrid mapping	(I) DNA can be cut into large fragments and circularized for use in chromosome walking
(B) Sequence tagged site (STS) mapping	(II) Useful for cloning of overlapping DNA fragments (restricted to about 200 kb)
(C) Chromosome jumping	(III) Fragment genome into large pieces and locate markers and genes
(D) Chromosome walking	(IV) Applicable to any part of DNA sequence if some sequence information is available

Choose the correct answer from the options given below:

• (A) - (I), (B) - (II), (C) - (III), (D) - (IV)
• (A) - (I), (B) - (III), (C) - (II), (D) - (IV)
• (A) - (I), (B) - (II), (C) - (IV), (D) - (III)
• (A) - (III), (B) - (IV), (C) - (I), (D) - (II)

Hint:

Understand the application of various mapping techniques and their significance in genome analysis.

Answer 1

The Correct Option is D

- Radiation hybrid mapping allows researchers to bypass DNA regions to locate distant markers and genes.
- Sequence tagged site (STS) mapping is applied to study DNA regions when sequence information is available.
- Chromosome jumping breaks DNA into large fragments, which are then circularized for chromosome walking.
- Chromosome walking is crucial for cloning overlapping DNA fragments.