Match LIST-I with LIST-II Choose the correct answer from the options given below:
Show Hint
Think of replication as road construction. \textbf{Helicase} is the bulldozer that separates the lanes. \textbf{Topoisomerase} is the crew that manages traffic jams (supercoils) up ahead. \textbf{DNA Pol III} is the paver laying down the new road (DNA). \textbf{DNA Pol I} is the repair crew that replaces the temporary starting cones (RNA primers) with permanent pavement (DNA).
Step 1: Concept Summary:
This question tests your knowledge of bacterial DNA replication enzymes and proteins and their functions. Step 2: Detailed Solution:
Match each protein from LIST-I with its corresponding function from LIST-II:
A. Helicase: This enzyme unwinds the DNA double helix at the replication fork, using ATP. It corresponds to II. Unwinds parental double helix at replication forks.
B. DNA pol I: This polymerase removes RNA primers (using 5' to 3' exonuclease activity) and replaces them with DNA (using 5' to 3' polymerase activity). It corresponds to I. Removes RNA nucleotides of primer...and replaces them with DNA nucleotides....
C. DNA pol III: The primary DNA replicase in bacteria, it rapidly synthesizes new DNA strands by adding nucleotides to the 3' end of a primer using the parental strand as a template. It corresponds to IV. Using parental DNA as a template, synthesis new DNA strand by adding nucleotides....
D. Topoisomerase (DNA gyrase): This enzyme relieves the torsional strain caused by DNA unwinding by cutting, unswiveling, and resealing the DNA. It corresponds to III. Relieves overwinding strain ahead of replication forks....
Step 3: Final Answer:
The correct matches are A-II, B-I, C-IV, D-III.
