List of top Biology Questions on Molecular Biology

Given below are two statements:
Statement I: The DNA fragments extracted from gel electrophoresis can be used in the construction of recombinant DNA.
Statement II: Smaller size DNA fragments are observed near the anode while larger fragments are found near the wells in an agarose gel.

Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase

The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce color in the presence of a chromogenic substrate. Given below are two statements about this method: Statement I: The blue-colored colonies have DNA insert in the plasmid, and they are identified as recombinant colonies. Statement II: The colonies without blue color have DNA insert in the plasmid, and are identified as recombinant colonies.

Following are the steps involved in the process of PCR.
a. Annealing
b. Amplification (~1 billion times)
c. Denaturation
d. Treatment with Taq polymerase and deoxynucleotides
e. Extension
Choose the correct sequence of steps of PCR from the options given below :

Given below are two statements
Statement I : RNA interference takes place in all Eukaryotic organisms as method of cellular defense.
Statement II : RNAi involves the silencing of a specific mRNA due to a complementary single-stranded RNA molecule that binds and prevents translation of mRNA
In the light of the above statements, choose the correct answer from the options given below

Which of the following are correct about EcoR1?
A. Cut the DNA with blunt end
B. Cut the DNA with sticky end
C. Recognise a specific palindromic sequence
D. Cut the DNA between the base G and A when encounters the DNA sequence ‘GAATTC’
E. Exonuclease
Choose the correct answer from the options given below:

Given below are two statements :
Statement I: In the lac operon, the z gene codes for beta-galactosidase which is primarily responsible for the hydrolysis of lactose into galactose and glucose.
Statement II: In addition to lactose, glucose or galactose can also induce lac operon.
In the light of the above statements, choose the correct answer from the options given below :

Which of the following are correct about cellular respiration?
A. Cellular respiration is the breaking of C-C bonds of complex organic molecules by oxidation.
B. The entire cellular respiration takes place in Mitochondria.
C. Fermentation takes place under anaerobic condition in germinating seeds.
D. The fate of pyruvate formed during glycolysis depends on the type of organism also.
E. Water is formed during respiration as a result of O2 accepting electrons and getting reduced
Choose the correct answer from the options given below:

Which of the following is the correct match?