Question

Which of the following are correct about EcoR1?
A. Cut the DNA with blunt end
B. Cut the DNA with sticky end
C. Recognise a specific palindromic sequence
D. Cut the DNA between the base G and A when encounters the DNA sequence ‘GAATTC’
E. Exonuclease
Choose the correct answer from the options given below:

• A. B, C, E only
• B. A, D, E only
• C. A, C, D only
• D. B, C, D only

Answer 1

The Correct Option is D

EcoRI, a restriction endonuclease, cleaves the palindromic sequence GAATTC between G and A, yielding sticky ends. It does not produce blunt ends or function as an exonuclease.