Question

Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase

• A. A and B only
• B. D and E only
• C. B and C only
• D. C and D only

Hint:

Gene cloning essentials: Cut (restriction enzymes), Paste (ligase), Copy (polymerase).

Answer 1

The Correct Option is D

Gene cloning utilizes restriction enzymes for DNA cutting, DNA ligase for fragment joining, and DNA polymerase for DNA copying. DNA mutase and DNA recombinase are not standard or essential enzymes for gene cloning with plasmid vectors. DNA polymerase is crucial for replicating the cloned DNA within the host.