Step 1: Understanding the Problem:
Various reagents and enzymes are employed during the different steps of recombinant DNA technology to isolate, visualize, and manipulate nucleic acids.
Step 2: Approach and Formula:
Match the specific molecular biology procedures with their corresponding chemical reagents or enzymatic agents.
Step 3: Detailed Explanation:
- a) Visualization of DNA: DNA fragments separated on an agarose gel cannot be seen under visible light. They are stained with Ethidium bromide and viewed under UV light, appearing as bright orange bands. (a $\rightarrow$ iv)
- b) Precipitation of DNA: After extracting DNA from the cell and removing proteins/RNA, the highly purified DNA is ultimately precipitated out of the aqueous solution by the addition of chilled ethanol. (b $\rightarrow$ iii)
- c) Breaking of cell wall of bacteria: To extract DNA from a bacterial cell, its peptidoglycan cell wall is broken down using the enzyme Lysozyme. (c $\rightarrow$ ii)
- d) Breaking of cell wall of fungus: Fungal cell walls are made of chitin, which is degraded by the specific enzyme Chitinase to release the internal genetic material. (d $\rightarrow$ i)
Matching all options: a-iv, b-iii, c-ii, d-i.
Step 4: Final Answer:
The correct matching sequence is (1).