Question

Which one of the following statements is correct in the context of observing DNA separation by agarose gel electrophoresis?

• A. DNA can be seen in visible light.
• B. DNA can be seen without staining in visible light.
• C. Ethidium bromide stained DNA can be seen in visible light.
• D. Ethidium bromide stained DNA can be seen under UV light.

Hint:

Ethidium bromide is widely used in molecular biology for visualizing DNA under UV light during gel electrophoresis.

Answer 1

The Correct Option is D

Step 1: Function of ethidium bromide.
- Ethidium bromide serves as a DNA stain in agarose gel electrophoresis. 
- Its intercalation into DNA facilitates visualization of DNA bands under UV illumination.