Question

Where does EcoRI cut the palindrome? Write the events followed thereafter to form a recombinant DNA.

Hint:

EcoRI is a restriction enzyme widely used in genetic engineering for creating recombinant DNA molecules.

Answer 1

EcoRI cleaves DNA between guanine (G) and adenine (A), creating staggered ends. Subsequently: Complementary base pairing facilitates the annealing of vector and foreign DNA sticky ends. DNA ligase repairs strand breaks, resulting in recombinant DNA.