Question

Liquid chromatography can be performed in which of the following ways?

• A. Only in columns
• B. Only on plane surfaces
• C. Either in columns or on plane surfaces
• D. Neither in columns nor on plane surfaces

Hint:

To remember this, think of the two "extremes": HPLC uses a metal Column for complex industrial analysis, while a simple lab student might use a flat Paper strip for a basic ink separation. Both are forms of liquid chromatography!

Answer 1

The Correct Option is C

1. Column Chromatography: The most common form is column chromatography, where the stationary phase is packed into a cylindrical tube (column). The mobile phase flows through this column under gravity or high pressure (as in HPLC). This method is highly effective for both analytical and preparative separations.

2. Planar Chromatography: Liquid chromatography can also be performed on a flat, "plane" surface.

• Paper Chromatography: The stationary phase is the water molecules trapped in the cellulose fibers of the paper.

• Thin-Layer Chromatography (TLC): The stationary phase is a thin layer of adsorbent material (like silica gel) coated onto a glass, plastic, or aluminum plate.