Question

Given below are two statements.
Statement I: Restriction enzyme BamHI has its recognition site in tet$^{R}$ region of pBR322.
Statement II: E.coli having pBR322 with a desired DNA if inserted at BamHI site can grow in medium containing tetracycline.
In the light of the above statements, choose the correct answer from the options given below.

• A. Both Statement I and Statement II are true
• B. Both Statement I and Statement II are false
• C. Statement I is true, but Statement II is false
• D. Statement I is false, but Statement II is true

Hint:

Insertional inactivation means putting a gene inside a resistance gene destroys that resistance. Insertion in BamHI $\rightarrow$ loss of Tetracycline resistance.

Answer 1

The Correct Option is C