Enhanced and Secure Single-tube DNA Sequencing:
Single-tube sequencing offers improvements in quality and safety due to its automation, accelerated processing time, and the utilization of a single lane for gel electrophoresis, thereby streamlining the procedure. Furthermore, it employs fluorescent dyes, which represent a safer alternative to the radioactive isotopes used in the initial methodology. The output can be directly visualized on a computer, facilitating simpler data storage.