List of top Biotechnology Questions on Genomics and DNA Sequencing asked in CBSE CLASS XII

What is a “metagenome”? Describe the metagenomics approach and write its importance to study microorganisms.

A comparative microarray hybridization experiment was performed between normal and cancerous cells. How will you interpret the result obtained on a microarray, if red and green coloured fluors were used for labelling the cDNA probes of normal and cancerous cells respectively?

With a suitable example, explain briefly, why the number of predicted genes do not correlate with the genome size and the number of chromosomes in an organism.

Single base difference in ApoE gene is associated with:

Briefly write the steps of Sanger’s chain termination method of DNA sequencing.

Why is single tube DNA sequencing considered better and safer ?

During DNA sequencing, why is the autoradiogram read from bottom to top to arrive at the original sequence ?

In Sanger's chain termination method, incorporation of ddNTP cause the growing DNA chains to terminate prematurely. Explain how.
(ii) Briefly write the steps of Sanger's chain termination method of DNA sequencing.