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Which purification method is commonly used when recombinant proteins carry a His-tag?

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His-tagged proteins are commonly purified using \[ \boxed{\text{Immobilized Metal Affinity Chromatography (IMAC)}} \] where the tag binds to \[ \boxed{\mathrm{Ni^{2+}} \text{ or } \mathrm{Co^{2+}}.} \]

Updated On: Jul 14, 2026

Ion exchange chromatography
Gel filtration chromatography
Affinity chromatography
Ultracentrifugation

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The Correct Option is C

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Which purification method is commonly used when recombinant proteins carry a His-tag?

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The Correct Option is C

Solution and Explanation

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