Question

Which one is the correct product of DNA dependent RNA polymerase to the given template?
3’TACATGGCAAATATCCATTCA5’

• A. 5’AUGUACCGUUUAUAGGUAAGU3’
• B. 5’AUGUAAAGUUUAUAGGUAAGU3’
• C. 5’AUGUACCGUUUAUAGGGAAGU3’
• D. 5’ATGTACCGTTTATAGGTAAGT3’

Answer 1

The Correct Option is A

Transcription of the DNA template strand 3’TACATGGCAAATATCCATTCA5’ is required to identify the product of DNA-dependent RNA polymerase. Base pairing rules dictate the mRNA sequence: adenine (A) pairs with uracil (U), thymine (T) pairs with adenine (A), cytosine (C) pairs with guanine (G), and guanine (G) pairs with cytosine (C). The resulting mRNA sequence transcribed from the 3’ to 5’ DNA strand is:

DNA	-	T	A	C	A	T	G	G	C	A	A	A	T	A	T	C	C	A	T	T	C	A
RNA	-	A	U	G	U	A	C	C	G	U	U	U	A	U	A	G	G	U	A	A	G	U

The transcribed RNA sequence is: 5'AUGUACCGUUUAUAGGUAAGU3'.
Therefore, the DNA-dependent RNA polymerase product for the given template is 5'AUGUACCGUUUAUAGGUAAGU3'.