Question

Which of the following statements are correct with respect to DNA separation, isolation and visualization ?
A. The cutting of DNA is done by molecular scissors.
B. The DNA fragments separate according to their size in an agarose gel, upon electrophoresis.
C. The separated DNA fragments can be seen without staining when exposed to UV light.
D. The separated DNA fragments, when stained with ethidium bromide, can be seen in visible light. Choose the correct answer from the options given below :

• A. B and C only
• B. B and D only
• C. A and B only
• D. A and D only

Hint:

For visualization, remember: EtBr + UV = Orange Bands. You cannot skip either the stain or the specific light source!

Answer 1

The Correct Option is C

Step 1 : Understanding the Question
Verify biotech procedures for DNA handling.
Step 2 : Key Formulas and approach
Evaluate the cutting mechanism and the staining requirements for visualization.
Step 3 : Detailed Explanation

Statement A: Correct. Enzymes cut DNA.

Statement B: Correct. Agarose sieves by size.

Statement C: Incorrect. Staining is mandatory.

Statement D: Incorrect. UV light is mandatory with EtBr.

Step 4 : Final Answer
Only A and B are correct (Option B).