Question

The production of haploids by anther and pollen culture was first demonstrated by -

• A. Maheswari and Guha
• B. Ravi and Chan
• C. Kasha and Kao
• D. Clausen and Cameron

Hint:

For competitive exams in biology and biotechnology, it's crucial to remember the names of key scientists associated with major discoveries, such as Mendel (genetics), Watson and Crick (DNA structure), and in this case, Maheswari and Guha (anther culture).

Answer 1

The Correct Option is A

Step 1: Concept Overview:
This question concerns a pivotal historical achievement in plant tissue culture. It requires identifying the scientists who pioneered the successful generation of haploid plants via anther and pollen culture methods.
Step 2: In-Depth Analysis:
Sipra Guha and Satish C. Maheshwari, two Indian scientists, are credited with the pioneering work on haploid production through anther in vitro culture.
In 1964, they documented the direct formation of embryos from Datura innoxia microspores (thorn apple) by culturing excised anthers.
This finding marked a key advancement in plant biotechnology, providing a quick pathway for creating homozygous diploid lines by doubling the chromosomes of haploids, greatly accelerating breeding processes.
The other listed scientists are recognized for different contributions, not this specific breakthrough.
Step 3: Conclusion:
Maheswari and Guha first demonstrated haploid production via anther culture.