Gene Overexpression: A method involves overexpressing genes responsible for the initial enzyme in a biosynthetic pathway. This initial enzyme frequently dictates the reaction rate; therefore, enhancing its activity boosts the production of the final product.
Agrobacterium-mediated Secondary Root Induction: Alternatively, *Agrobacterium rhizogenes* can be employed to stimulate the excessive development of secondary roots in plants that naturally synthesize valuable secondary metabolites within these roots, thereby increasing both production and yield.