Question

The DNA ligase enzyme that is frequently used to ligate different DNA fragments in order to generate rDNA molecules is isolated from:

• A. T2 Bacteriophage
• B. T4 Bacteriophage
• C. Lambda Bacteriophage
• D. M13 filamentous phage

Answer 1

The Correct Option is B

DNA ligase, a crucial enzyme in recombinant DNA technology, facilitates the joining of DNA fragments by catalyzing the formation of phosphodiester bonds. The T4 bacteriophage is the source of the DNA ligase most commonly employed in laboratory settings.

• T4 Bacteriophage: This phage yields T4 DNA ligase, a highly efficient enzyme capable of ligating both sticky and blunt DNA ends, making it the standard for recombinant DNA molecule construction in genetic engineering.
• T2 Bacteriophage: While similar, it is not the origin of the widely used DNA ligase.
• Lambda Bacteriophage: Primarily utilized as a cloning vector, it does not supply DNA ligase.
• M13 Filamentous Phage: This phage plays a role in sequencing and cloning vectors but does not produce the ligase enzyme.

Consequently, the most appropriate selection is (B) T4 Bacteriophage.