Question

The correct order of steps in the process of recombinant DNA technology are:
1. Digestion by restriction enzyme
2. Transformation
3. Ligation
4. Isolation of DNA

• A. (D), (C), (B), (A)
• B. (A), (C), (B), (D)
• C. (D), (A), (C), (B)
• D. (A), (D), (C), (B)

Hint:

Recombinant DNA technology requires precise execution of steps in the correct order to achieve the desired genetic modification.

Answer 1

The Correct Option is C

The process of recombinant DNA technology follows these steps:
1. DNA Isolation (D): DNA is obtained from the source organism.
2. Restriction Digestion (A): Specific enzymes cut the DNA at target sites.
3. Ligation (C): DNA fragments are joined using DNA ligase.
4. Transformation (B): Recombinant DNA is introduced into a host for replication and expression.
This sequence ensures the proper preparation and successful introduction of recombinant DNA into the host.