Question

Match List I with List II : 

List I	List II
A. Genetically modified organism  B. Thermostable DNA polymerase  C. Ti plasmid  D. pBR322	I. Agrobacterium tumefaciens  II. Bt cotton  III. Thermus aquaticus  IV. Escherichia coli

Choose the correct answer from the options given below :

• A. A-II, B-III, C-I, D-IV
• B. A-I, B-IV, C-III, D-II
• C. A-I, B-II, C-IV, D-III
• D. A-II, B-I, C-IV, D-III

Hint:

Associate \textbf{T}hermostable with {\textbf{T}hermus aquaticus} and \textbf{Ti} plasmid with {\textbf{A}grobacterium} (The Natural Genetic Engineer).

Answer 1

The Correct Option is A

Step 1: Understanding the Question
The task is to correctly match biological entities or tools from List I with their corresponding examples or sources in List II. This involves knowledge of biotechnology, specifically concerning cloning vectors, genetically modified crops, and enzymatic tools used in molecular biology. Step 2: Key Formula or Approach
To solve this match-the-following question, analyze each item in List I and find its standard biological association in List II.
- A GMO is an organism whose DNA has been altered.
- Thermostable enzymes are needed for PCR.
- Ti plasmids are "natural genetic engineers."
- pBR322 is a common lab vector.
Step 3: Detailed Explanation
A. Genetically modified organism (GMO): {Bt} cotton is a commercially grown GM crop that contains a toxin gene from {Bacillus thuringiensis} to resist bollworms. Thus, A matches with II.
B. Thermostable DNA polymerase: Taq polymerase is used in PCR because it doesn't denature at high temperatures. It is isolated from the hot-spring bacterium {Thermus aquaticus}. Thus, B matches with III.
C. Ti plasmid: The Tumor-inducing (Ti) plasmid is found in the soil bacterium {Agrobacterium tumefaciens}. It is used as a vector to transfer DNA into plant cells. Thus, C matches with I.
D. pBR322: This is one of the most widely used synthetic cloning vectors in genetic engineering. It was designed to work and replicate inside {Escherichia coli}. Thus, D matches with IV.
Step 4: Final Answer
The correct combination is A-II, B-III, C-I, D-IV. Comparing this with the given options, (A) is the correct answer.