Concept:
For a quadratic equation \(ax^2+bx+c=0\) with roots \(\alpha, \beta\):
\[
\alpha+\beta = -\frac{b}{a}, \qquad \alpha\beta = \frac{c}{a}
\]
Also,
\[
\frac{1}{\alpha}-\frac{1}{\beta}=\frac{\beta-\alpha}{\alpha\beta}
\]
Step 1: Find sum and product of roots.
Given:
\[
\lambda x^2-(\lambda+3)x+3=0
\]
\[
\alpha+\beta=\frac{\lambda+3}{\lambda}, \qquad \alpha\beta=\frac{3}{\lambda}
\]
Step 2: Use the given condition.
\[
\frac{1}{\alpha}-\frac{1}{\beta}
=\frac{\beta-\alpha}{\alpha\beta}
=\frac{1}{3}
\]
\[
\Rightarrow \frac{\beta-\alpha}{\frac{3}{\lambda}}=\frac{1}{3}
\Rightarrow \beta-\alpha=\frac{1}{\lambda}
\]
Step 3: Express \(\beta-\alpha\) using sum and product.
\[
(\beta-\alpha)^2=(\alpha+\beta)^2-4\alpha\beta
\]
\[
(\beta-\alpha)^2
=\left(\frac{\lambda+3}{\lambda}\right)^2-\frac{12}{\lambda}
=\frac{(\lambda-3)^2}{\lambda^2}
\]
\[
\Rightarrow \beta-\alpha=\frac{|\lambda-3|}{\lambda}
\]
From Step 2:
\[
\frac{|\lambda-3|}{\lambda}=\frac{1}{\lambda}
\Rightarrow |\lambda-3|=1
\]
Step 4: Find possible values of \(\lambda\).
\[
\lambda-3=1 \Rightarrow \lambda=4
\]
\[
\lambda-3=-1 \Rightarrow \lambda=2
\]
Step 5: Find the required sum.
\[
\lambda_1+\lambda_2=4+2=6
\]
Conclusion:
\[
\boxed{\text{Sum of all possible values of } \lambda = 6}
\]
Hence, the correct answer is (4).