Question:medium

Given below is a single stranded sequence of DNA:
5' ACTAGAATTCGCCA 3'
Which restriction enzyme would cleave this sequence in double stranded form? Illustrate the fragments that will be generated upon digestion by this restriction enzyme.

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EcoRI cuts specifically between G and A in the palindromic sequence $5'\text{--GAATTC--}3'$. The resulting sticky ends can hydrogen-bond with any other DNA fragment cut by the same enzyme, which is then sealed covalently by DNA ligase.
Updated On: Jun 19, 2026
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