Question

Given below are two statements:
Statement (I): In a standard PCR amplification with a co-dominant marker, a pair of primers (Forward and Reverse) is used.
Statement (II): In a standard PCR, only the segment of DNA whose 3'-ends are complementary to the two primers used only gets amplified.
In light of the above statements, choose the most appropriate answer from the options given below:

• A. Both Statement (I) and Statement (II) are correct.
• B. Both Statement (I) and Statement (II) are incorrect.
• C. Statement (I) is correct but Statement (II) is incorrect.
• D. Statement (I) is incorrect but Statement (II) is correct.

Hint:

Remember that DNA polymerase can only synthesize DNA in the 5' to 3' direction.
Because it requires a free 3'-OH group to initiate synthesis, the 3' ends of the forward and reverse primers must point toward each other to amplify the region between them.

Answer 1

The Correct Option is A