Question

Find the correct sequence of reaction catalyzed by DNA ligase: A. DNA ligase ligates Okazaki fragment to rest of lagging strand. B. Polymerase I eliminates downstream RNA primer by nick translation. C. Unwinding of parental duplex by helicase and elongation of leading strand by polymerase III exposes single-stranded region in front of lagging strand. D. Polymerase III extends DNA Okazaki fragments from primer. E. Primase synthesizes RNA primer.

• A. C, E, D, B, A
• B. E, A, D, C, B
• C. B, A, C, E, D
• D. B, C, A, D, E

Hint:

Lagging strand synthesis sequence: helicase unwinds DNA, primase makes primer, DNA polymerase III extends, DNA polymerase I removes primer, and ligase seals the nick.

Answer 1

The Correct Option is A