Question

CRISPR-Cas 9 is a gene __________ technique.

• A. sequencing
• B. labelling
• C. editing
• D. locating

Hint:

Associate {CRISPR} with the concept of "molecular scissors" or a "find-and-replace" function for DNA. This directly points to its role in gene {editing}.

Answer 1

The Correct Option is C

Step 1: Concept Overview:
The objective is to categorize the CRISPR-Cas9 system by its core molecular biology function.
Step 2: In-Depth Explanation:
CRISPR-Cas9, originating from bacterial defense mechanisms, is a transformative technology enabling precise DNA modifications in living organisms.
The CRISPR component functions as a guide RNA, targeting and binding to a specific DNA sequence.
The Cas9 component, a nuclease enzyme often called "molecular scissors," cleaves the DNA at the designated site.
This DNA cleavage allows for the removal, addition, or alteration of specific DNA sequences, a process known as gene editing, or targeted genome modification.
Sequencing involves determining the precise nucleotide order in a DNA molecule.
Labelling is the attachment of a marker (e.g., fluorescent dye) to a molecule for detection.
Locating is a component of the process, but the primary function is gene alteration, not simply location.
Step 3: Conclusion:
CRISPR-Cas9 is a potent tool for gene editing.