Question

Chromatographic technique/s based on the principle of differential adsorption is/are
A. Column chromatography
B. Thin layer chromatography
C. Paper chromatography
Choose the most appropriate answer from the options given below:

• A. B only
• B. A only
• C. A & B only
• D. C only

Answer 1

The Correct Option is C

Chromatography is a separation technique for mixtures. It works by exploiting differences in component affinities for a stationary phase as they are moved by a mobile phase. Differential adsorption is one such separation principle used in chromatography.

1. Differential Adsorption Explained:
   • This principle relies on how strongly different mixture components bind to a stationary phase.
   • Components with stronger adsorption to the stationary phase move slower, enabling separation from those with weaker adsorption.
2. Evaluation of Methods:
   • Column Chromatography (A):
     • This method uses a stationary phase packed in a column. Separation occurs due to differential adsorption onto this phase.
     • Compounds interacting more strongly with the stationary phase progress slower through the column.
   • Thin Layer Chromatography (B):
     • TLC also employs differential adsorption for separation.
     • A thin layer of adsorbent on a plate forms the stationary phase, and separation is driven by interactions with this adsorbent.
   • Paper Chromatography (C):
     • This technique is primarily based on partition, not adsorption.
     • Separation depends on solubility differences, with the paper acting as a stationary phase that holds a liquid stationary phase.
3. Summary:
   • Both Column Chromatography and Thin Layer Chromatography operate using the principle of differential adsorption.
   • Consequently, the correct selection is A & B only.