Question

Choose the correct statements regarding plant tissue culture -
A. Organogenesis is inducing the formation of various vegetative organs from cells or tissues.
B. Formation of mass of undifferentiation cells from callus redifferentiation.
C. Cytoplasmic hybrids are prepared by taking the nucleus from one parent and cytoplasm from both the parents.
D. Relative concentration of growth hormones play important role in organogenesis.
Choose the correct answer from the options given below :

• A. A, B, and C Only
• B. B, C and D Only
• C. A and B Only
• D. A, C and D Only

Hint:

Remember the auxin-to-cytokinin ratio rule: High Auxin/Cytokinin ratio generally induces root formation, while a Low Auxin/Cytokinin ratio induces shoot formation.

Answer 1

The Correct Option is D

Step 1: Concept Overview:
The question assesses the validity of four statements regarding plant tissue culture.
Step 2: Detailed Analysis:
A. Organogenesis involves inducing the formation of vegetative organs from cells or tissues. This is an accurate definition of organogenesis in plant tissue culture. (Correct)
B. Formation of an undifferentiated cell mass from callus redifferentiation. This statement is incorrect. Dedifferentiation is the process where differentiated explants form an undifferentiated callus. Redifferentiation is the formation of organs from the callus. The statement reverses these processes. (Incorrect)
C. Cytoplasmic hybrids are created by combining the nucleus of one parent with the cytoplasm of both parents. This accurately describes cybrid creation. It's typically done by fusing a normal protoplast (nucleus + cytoplasm) from one parent with an enucleated protoplast (cytoplasm only) from another, resulting in a cell with one parent's nucleus and mixed cytoplasm. (Correct)
D. Growth hormone concentrations significantly influence organogenesis. This is a key principle in plant tissue culture. The auxin to cytokinin ratio determines if the callus differentiates into roots, shoots, or remains undifferentiated. (Correct)
Step 3: Conclusion:
Statements A, C, and D are correct. Therefore, the correct option is A, C and D Only.