Step 1: Concept Overview:
This question concerns doubled haploids: homozygous diploid plants utilized in plant breeding.
Step 2: Detailed Analysis:
A. Production via Haploid Chromosome Doubling: Doubled haploids are created by duplicating the chromosome number of haploid plants. Haploids (n) are generated through anther or ovule culture, followed by chromosome doubling to yield a completely homozygous diploid (2n) plant. (Correct)
B. Recessive Trait Expression After Doubling: Haploid organisms express all genes, including recessive alleles, due to the absence of a masking allele. Chromosome doubling (e.g., 'r' becoming 'rr') fixes the recessive trait in a homozygous state, maintaining its expression. (Correct)
C. Chromosome Doubling via Ozone: Ozone, a strong oxidizing agent, is used for sterilization, not for inducing polyploidy. This statement is (Incorrect).
D. Chromosome Doubling via Colchicine: Colchicine, a chemical from the autumn crocus, is a mitotic inhibitor. It disrupts spindle fiber formation during cell division, preventing chromosome separation and causing chromosome number doubling. This is the standard method for producing doubled haploids. (Correct)
Step 3: Conclusion:
Statements A, B, and D are correct.