Question

Arrange the following steps in the process of somatic hybridization in correct sequence:
A. Plating of fused protoplasts
B. Selection of hybrid cells
C. Protoplast isolation and its treatment with fusion chemical.
D. Transfer of callus to differentiation medium
E. Selection of somatic hybrid plants
Choose the correct answer from the options given below :

• A. A, B, C, D, E
• B. C, A, B, D, E
• C. B, C, D, A, E
• D. A, C, B, D, E

Hint:

Think of the process from small to big: {Isolate} cells (C) \(\rightarrow\) {Plate} and grow them (A) \(\rightarrow\) {Select} the right cells (B) \(\rightarrow\) {Differentiate} into a plant (D) \(\rightarrow\) {Select} the best plant (E).

Answer 1

The Correct Option is B

Step 1: Concept Overview:
The problem requires sequencing the steps of somatic hybridization, a method for creating hybrid plants by fusing somatic cells.
Step 2: Process Breakdown:
The correct sequence is:
C. Protoplast isolation and fusion treatment: Isolate cells and remove cell walls to form protoplasts. Treat with a fusogen (e.g., PEG) to induce fusion.
A. Plating fused protoplasts: Plate the mixture of fused and unfused protoplasts on a culture medium to enable cell wall regeneration and division.
B. Hybrid cell selection: Select hybrid cells (heterokaryons) from parental cells and homokaryons using appropriate methods.
D. Callus transfer to differentiation medium: Grow selected hybrid cells into a callus. Transfer the callus to a medium with hormones to induce shoot and root formation (organogenesis).
E. Somatic hybrid plant selection: Select regenerated plantlets to confirm they are true hybrids with the desired traits.
This yields the order: C \(\rightarrow\) A \(\rightarrow\) B \(\rightarrow\) D \(\rightarrow\) E.
Step 3: Solution:
The correct somatic hybridization sequence is C, A, B, D, E.