Question

Arrange the following steps for plant tissue culture (P.T.C.) in correct sequence:
A. Selection of desired material and suitable nutrient media for P. T. C.
B. Inoculation of explants
C. Surface sterilization of explant
D. Transfer of growing cultures
E. Transfer of plantlets to soil in pots
Choose the correct answer from the options given below :

• A. A, B, C, D, E
• B. A, C, B, D, E
• C. A, C, E, B, D
• D. B, C, A, E, D

Hint:

Remember the logic of asepsis in biology labs: {Prepare} (A), {Sterilize} (C), then {Inoculate} (B). Never inoculate before sterilizing the explant. The final step is always moving the plantlet out of the lab and into the soil (E).

Answer 1

The Correct Option is B

Step 1: Concept Overview:
The problem requires ordering the general steps of a plant tissue culture experiment, from start to plant establishment.
Step 2: Step-by-Step Analysis:
Let's break down the steps for a logical sequence:
A. Material and Media Selection: This initial stage involves choosing the plant material (explant) and preparing the sterile nutrient medium.
C. Explant Sterilization: To prevent contamination, the explant's surface must be sterilized before introduction to the sterile culture.
B. Explant Inoculation: The sterilized explant is then placed on or in the sterile nutrient medium within a culture vessel.
D. Culture Transfer: As cultures grow, nutrients deplete, necessitating periodic transfer (subculturing) to fresh medium during the in vitro phase.
E. Plantlet Transfer to Soil: Once a plantlet develops roots and shoots in vitro, it's moved to soil, a process of hardening or acclimatization.
Therefore, the correct order is A \(\rightarrow\) C \(\rightarrow\) B \(\rightarrow\) D \(\rightarrow\) E.
Step 3: Conclusion:
The plant tissue culture steps in the correct order are A, C, B, D, E.