Question

A researcher performed protein fingerprinting on hemoglobin from both normal and sickled red blood cells. Complete the flow-chart of the process by filling A, B, and C.

 

Hint:

Be clear about the sequence of steps used in protein fingerprinting and the type of information that can be gained from each step.

Answer 1

A - Trypsin Digestion: Proteins undergo initial treatment with trypsin, a proteolytic enzyme that cleaves them at defined amino acid sites.

B - Electrophoretic Separation: Subsequent paper electrophoresis separates the generated protein fragments according to their electrical charge.

C - Peptide Sequencing: The final stage involves sequencing non-overlapping peptides eluted from chromatography, thereby determining their individual amino acid order.