List of top Plant Biotechnology Questions on Plant Biotechnology

A microbial biocontrol agent which control butterfly caterpillars in plants is -

Arrange the following steps in the process of somatic hybridization in correct sequence:
A. Plating of fused protoplasts
B. Selection of hybrid cells
C. Protoplast isolation and its treatment with fusion chemical.
D. Transfer of callus to differentiation medium
E. Selection of somatic hybrid plants
Choose the correct answer from the options given below :

Haploids can be artificially produced by -
A. Colchicine doubling
B. X-ray treatment
C. Pollen culture
D. Distant hybridization
E. Infrared radiation
Choose the correct answer from the options given below :

Optimum pH for protoplast culture is -

'Pomato' a hybrid of potato and tomato was produced through-

Source organism of cry genes is -

In plant tissue culture formation of organ primordia-like shoot or root in callus cells is called -

K. J. Kasha and coworkers found that following the cross between Hordeum vulgare \(\times\) Hordeum bulbosum, chromosomes of H. bulbosum were eliminated in early zygotic division, so few days after pollination, embryos can be cultured to get haploids. This method is called as -

Choose the correct statements regarding plant tissue culture -
A. Organogenesis is inducing the formation of various vegetative organs from cells or tissues.
B. Formation of mass of undifferentiation cells from callus redifferentiation.
C. Cytoplasmic hybrids are prepared by taking the nucleus from one parent and cytoplasm from both the parents.
D. Relative concentration of growth hormones play important role in organogenesis.
Choose the correct answer from the options given below :

San Noeum first successfully cultured gynogenic haploid plants from unfertilized ovaries of -

Choose the correct statements regarding homozygous diploid plants -
A. They are produced by doubling the chromosome number of haploids.
B. Doubling of chromosome enables the recessive traits to express too.
C. Chromosome doubling is done by Ozone treatment.
D. Chromosome doubling is done by colchicine treatment.
Choose the correct answer from the options given below:

The process of combining cytoplasmic genomes of one parent with nuclear genome of other parent is called -

The cutting of DNA at specific locations became possible with the discovery of -

Select the correct statements regarding somaclonal variations
A. These variations can also result in unwanted traits.
B. Somaclones have been developed and proved advantageous in several crops.
C. These variations can be used to engineer novel traits.
D. Short term invitro callus and cell suspension, cultures results in somaclonal variation.
Choose the correct answer from the options given below :

CRISPR-Cas 9 is a gene __________ technique.

Virus free plants can be grown by : -

Which of the following methods/tools is not used for introduction of recombinant DNA into host cell?

Inducing the formation of various vegetative organs from cells or tissues in plant tissue culture is called -

The correct combination of somaclonal variant released as a new cultivar is -

Arrange the following steps for plant tissue culture (P.T.C.) in correct sequence:
A. Selection of desired material and suitable nutrient media for P. T. C.
B. Inoculation of explants
C. Surface sterilization of explant
D. Transfer of growing cultures
E. Transfer of plantlets to soil in pots
Choose the correct answer from the options given below :

Who is regarded as father of 'Plant Tissue Culture' (P. T. C.)?

The production of haploids by anther and pollen culture was first demonstrated by -

Production of secondary metabolites require the use of -

The first androgenic haploid plant product by anther culture was from -