List of top Plant Biotechnology Questions

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): The hybrid cells formed by the fusion of two unrelated diploid protoplasts combine a set of three genomes from the parents.
Reason (R): Partial or complete elimination of nuclear genome before regeneration leads to the formation of cybrid plants.
In the light of the above statements, choose the most appropriate answer from the options given below:

Select the correct sequence of steps involved in the isolation of protoplasts from the mesophyll cells of a plant leaf:
A. Culture of protoplast suspension
B. Infiltration of the enzyme into the leaf tissue
C. Incubate leaf pieces in filter-sterilized enzyme solution
D. Release of mesophyll protoplasts in solution
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Match List - I with List - II.

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Which factors do show considerable influence on the activity of nitrogenase enzyme during $N_{2}$ fixation?
A. Molybdenum
B. Molecular $H_{2}$
C. Boron
D. Leghemoglobin
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Match List - I with List - II.

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Match List - I with List - II.

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Which inorganic form(s) of nitrogen do plants assimilate?
A. Amino acids
B. $NH_{4}^{+}$
C. ${NO_{3}}^{-}$
D. ${NO_{2}}^{-}$
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Which are the symbiotic nitrogen-fixing bio-fertillizers?
A. Rhizobium
B. Azotobacter
C. Azospirillum
D. Azollae
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Find the correct order for the conversion of organic form of nitrogen into inorganic form by soil microorganisms.
A. Formation of nitrites
B. Formation of ammonia
C. Formation of free nitrogen
D. Formation of nitrates
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Mention the correct sequence of the formation of compounds during biological nitrogen fixation.
A. Hydrazine
B. Nitrogen
C. Ammonia
D. Diamide
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The steps involved in the process of soyabean root nodule formation are:
A. Bacteria emits signals to stimulate root hairs to elongate
B. Cortex and pericycle cells begin division
C. Roots emits signals to attract Rhizobium
D. Bacteria within vesicles develop into bacteroids
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): Manure is an organic substance that is obtained from decomposition of vegetable and animal waste.
Reason (R): Manure is readily soluble in water thus it is absorbed by plants quickly.
In the light of the above statements, choose the most appropriate answer from the options given below:

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): Uptake of hydrogenase activity is a mechanism of removing hydrogen from the site of nitrogen activity which concurrently carries out reactions of nitrogen fixation and hydrogen production.
Reason (R): Acetylene reduction technique is a common method of measuring nitrogenase activity.

The conversion of ammonia or ammonium ions to nitrite followed by its oxidation to nitrate is called as:

Which is not a free-living asymbiotic nitrogen fixation microorganism?

Which enzyme catalyzes the conversation of molecular $N_{2}$ to $NH_{3}$?

Which of the following is not a phosphate solubilizing microorganism?

Which of the following organism is a free living diazotroph?

Which of the soil bacteria convert ammonium into nitrite?

How many ATP molecules are required to produce two $NH_{3}$ molecules during nitrogen fixation?

Match List - I with List - II.

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Match List - I with List - II.

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Match List I with List - II.

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Nitrogen-fixing bacteria convert atmospheric nitrogen into which product?

Which of the following are used as osmotic stabilizers for the maintenance of protoplasts?
A. Glucose
B. Mannitol
C. PEG
D. Sorbitol
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