List of top Biotechnology Questions on Gene transfer technologies

Explain briefly the procedures of the following techniques used in recombinant DNA Technology:
(i) Transfection
(ii) Electroporation
(iii) Biolistics

On which plasmid are Bacterial Artificial Chromosome (BAC) vectors based? Enlist the important sequences present in these vectors.

What is meant by 'Gene knock out'? How is it useful in developing mouse models for studying human diseases?

SCID (Severe Combined Immuno Deficiency) is caused due to deficiency of which enzyme?

Mention the important features that were incorporated in each of the following vectors:
(i) COSMIDS
(ii) Shuttle Vectors
(iii) Expression Vectors

Absence of the enzyme adenosine deaminase in humans causes:

A biotechnologist wants to obtain a gene sequence in single stranded form. Which bacteriophage based vector should he choose to obtain the desired result?