Question

A biotechnologist wants to obtain a gene sequence in single stranded form. Which bacteriophage based vector should he choose to obtain the desired result?

• A. M13 based vector
• B. Bacteriophage Lambda based vector
• C. Plasmid cloning vector of pUC family
• D. Plasmid vector pBR 322

Hint:

M13 vectors are preferred for producing single-stranded DNA, useful in sequencing and mutagenesis.

Answer 1

The Correct Option is A

M13 bacteriophage-based vectors, which are filamentous phages, facilitate single-stranded DNA production in molecular cloning. This characteristic renders them optimal for applications like DNA sequencing and site-directed mutagenesis, both of which necessitate single-stranded DNA.

• M13 based vector: Generates single-stranded DNA, beneficial for gene sequencing.
• Lambda bacteriophage vector: Typically yields double-stranded DNA.
• Plasmid vectors (pUC family, pBR 322): These are circular double-stranded DNA vectors employed for cloning; however, they do not inherently produce single-stranded DNA.

Consequently, the appropriate selection is (A) M13 based vector.